In Vitro Shoot Induction of Musa acuminata cv. Mas Kirana

Raden Roro Rifka Annisa, Arkan Setiaji, Aries Bagus Sasongko


Abstract: The availability of ‘mother plants’ used for source of explants is one of the most important limitations for the development of in vitro culture in new banana cultivar’s. To increase produce large quantities of uniform and healthy seedlings, induction of multiplication has been carried out on Musa acuminata cv. Mas Kirana. Plant regeneration were obtained by culturing sucker on MS medium supplemented with PGRs BAP and NAA (1 mg/l + 0.5 mg/l, 2 mg/l + 0.5 mg/l, and 3 mg/l + 0.5 mg/l) and TDZ (0.25 mg/l, 0.5 mg/l, and 0.75 mg/l). The highest shoot formation was found in 0.25 mg/l TDZ with average shoots of 13.67 ± 3.16 and primary shoot height is 20 cm. TDZ induction tends to form somatic embryos, while induction of BAP and NAA leads to organogenesis. The rate of contaminants occurrence from bacteria is 87.50% and 12.50% from fungus. In histological observation, TDZ, BAP, and NAA affect the development of many new meristematic zones seen in the scalps.


shoot induction; Musa acuminata; sucker explant; organogenesis; in vitro culture

Full Text:



Aquil, B., A.T. Jan, N.B. Sarin, and Q.M.R. Haq. 2012. Micropropagation and genetic transformation of banana for crop improvement and sustainable agriculture. Journal of Crop Science, 3(2): 64-77.

Abeyaratne, W.M. and M.A. Lathiff. 2002. In vitro propagation of Rathambala (Musa AAA) and the occurrence of phenotypic variations in the pseudostem. Annals of the Sri Lanka Department of Agriculture (LKA), 4: 191-197.

Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

Chawla, H. 2009. Introduction to Plant Biotechnology (3/e). Boca Raton: CRC Press

Divakaran, S.P. and A.S. Nair. 2011. Somatic embryogenesis from bract cultures in diploid Musa acuminata cultivars from South India. Scientia horticulturae, 131: 99-102.

Elhory, S.M.A., M.A. Aziz, A.A. Rashid, and A.G. Yunus. 2009. Prolific plant regeneration through organogenesis from scalps of Musa sp cv. Tanduk. African Journal of Biotechnology, 8(22): 6208-6213.

Fraser, N.J., H. Hashimoto, and R.J. Cogdell. 2001. Carotenoids and bacterial photosynthesis: The story so far... Photosynthesis research, 70(3): 249-256.

Ganapathi, T.R., N.S. Higgs, P.J. Balint-Kurti, C.J. Arntzen, G.D. May and J.M. Van Eck. 2001. Agrobacterium-mediated transformation of embryogenic cell suspensions of the banana cultivar Rasthali (AAB). Plant Cell Reports, 20(2): 157-162.

Govindaraju, S., J. Saravanan, B. Jayanthi, D. Nancy, and I. Arulselvi. 2012. In vitro propagation of Banana (Musa sp-Rasthali variety) from sword suckers for its commercial production. Research in Plant Biology, 2(5).

Grapin, A., J.L. Ortiz, T. Lescot, N. Ferrière, and F.X. Côte. 2000. Recovery and regeneration of embryogenic cultures from female flowers of False Horn Plantain. Plant Cell, Tissue and Organ Culture, 61(3): 237-244.

Gübbük, H. and M. Pekmezci. 2004. In vitro propagation of some new banana types (Musa spp.). Turkish Journal of Agriculture and Forestry, 28(5): 355-361.

Habiba, S.U., K. Shimasaki, M.M. Ahasan, and M.M. Alam. 2014. Effect of different cytokinins on in vitro organogenesis in protocorm-like bodies (PLBs) of Epidendrum ‘Rouge Star No. 8’. Middle East J. Sci. Res, 21: 1843-7.

Jafari, N., R.Y. Othman, and N. Khalid. 2011. Effect of benzylaminopurine (BA) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan. African Journal of Biotechnology, 10(13): 2446-2450.

Jalil, M., N. Khalid, and R.Y. Othman. 2003. Plant regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA). Plant Cell, Tissue and Organ Culture, 75(3): 209-214.

Khalil, S., K. Cheah, E. Perez, D. Gaskill, and J. Hu. 2002. Regeneration of banana (Musa spp. AAB cv. Dwarf Brazilian) via secondary somatic embryogenesis. Plant Cell Reports, 20(12): 1128-1134.

Kuo, H.L., J.T. Chen, and W.C. Chang. 2005. Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis ‘Little Steve’. In Vitro Cellular & Developmental Biology-Plant, 41(4).

Mante, S. and H.B. Tepper. 1983. Propagation of Musa textilis Nee plants from apical meristem slices in vitro. Plant Cell, Tissue and Organ Culture, 2(2): 151-159.

Mok, D.W. and M.C. Mok. 2001. Cytokinin metabolism and action. Annual review of plant biology, 52(1): 89-118.

Msogoya, T., H. Kanyagha, J. Mutigitu, M. Kulebelwa, and D. Mamiro. 2012. Identification and management of microbial contaminants of banana in vitro cultures. Journal of Applied Biosciences, 55: 3987-3994.

Pérez-Hernández, J. B. and P. Rosell-García. 2008. Inflorescence proliferation for somatic embryogenesis induction and suspension-derived plant regeneration from banana (Musa AAA, cv.‘Dwarf Cavendish’) male flowers. Plant cell reports, 27(6): 965-971.

Prahardini, P.E.R. and B.P.T.P.J. Timur. 2018. Karakterisasi Varietas Unggul Pisang Mas Kirana dan Agung Semeru di Kabupaten Lumajang. Buletin Plasma Nutfah, 16(2): 126-133.

Rahman, M.M., M.G. Rabbani, M.A. Rahman, and M.F. Uddin. 2002. In vitro shoot multiplication and rooting of banana cv. Sabri. Pakistan Journal of Biological Sciences, 5(2): 161-164.

Rahman, M.Z., K.M. Nasiruddin, M.A. Amin, and M.N. Islam. 2004. In vitro response and shoot multiplication of banana with BA and NAA. Asian Journal of Plant Sciences, 3(4): 406-409.

Rayaprolu, S., G. Mathian, and G. Ranjitha. 2015. Can in vitro contaminated culture be revived-A case study with contaminated cultures of tuberose (Polianthes tuberosa). Curr. Biotica, 9, 285-288.

Remakanthan, A., T.G. Menon, and E.V. Soniya. 2014. Somatic embryogenesis in banana (Musa acuminata AAA cv. Grand Naine): effect of explant and culture conditions. In Vitro Cellular & Developmental Biology-Plant, 50(1): 127-136.

Resmi, L. and A.S. Nair. 2007. Plantlet production from the male inflorescence tips of Musa acuminata cultivars from South India. Plant cell, tissue and organ culture, 88(3): 333-338.

Sadik, K., P.R. Rubaihayo, M.J.S. Magambo, and M. Pillay. 2007. Generation of cell suspensions of East African highland bananas through scalps. African Journal of Biotechnology, 6(11): 1352-1357.

Saeedavi, L., A. Soleimani, and M.E. Amiri. 2017. Improvement of shoot-tip culture proliferation in banana using PEG 6000. Iranian Journal of Plant Physiology, 7(3).

Shirani, S., F. Mahdavi, and M. Maziah. 2009. Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BA from excised shoot tips. African Journal of Biotechnology, 8(21): 5755-5761.

Sholi, N.J., A. Chaurasia, A. Agrawal, and N.B. Sarin. 2009. ABA enhances plant regeneration of somatic embryos derived from cell suspension cultures of plantain cv. Spambia (Musa sp.). Plant Cell, Tissue and Organ Culture, 99(2): 133-140.

Siamak, S., S. Meon, Z. Wahab, and M. Mahmood. 2010. Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.). American Journal of Agricultural and Biological Sciences, 5(2): 128-134.

Sidha, M., P. Suprasanna, V.A. Baat, U.G. Kulkarni, and B.N. Shinde. 2007. Developing somatic embryogenic culture system and plant regeneration in banana. Bhabha At. Res. Centre (BARC) Newslett, 285, 153-161.

Singh, C.V. 2018. Review on problems and its remedy in plant tissue culture. Asian J. Biol. Sci, 11(4): 165-172.

Sipen, P. and M.R. Davey. 2012. Effects of N6-benzylaminopurine and indole acetic acid on in vitro shoot multiplication, nodule-like meristem proliferation and plant regeneration of Malaysian bananas (Musa spp.). Tropical life sciences research, 23(2).

Smitha, P.D., K.R. Binoy, and A.S. Nair. 2014. Effect of TDZ on direct shoot regeneration from whole male inflorescence of four diploid banana cultivars from South India. Plant Science International, 1, 24-32.

Steart Jr, C. N. (ed). 2nd Ed. 2016. Plant biotechnology and genetics: principles, techniques, and applications. New Jersey: John Wiley & Sons.

Stirk, W.A. and J. Van Staden. 2010. Flow of cytokinins through the environment. Plant Growth Regulation, 62(2): 101-116.

Strosse, H., I. Van den Houwe, and B. Panis. 2004. Banana cell and tissue culture-review. Banana improvement: Cellular, molecular biology, and induced mutations, 1-12.

Suprasanna, P., M. Sidha, and T.R. Ganapathi. 2008. Characterization of radiation induced and tissue culture derived dwarf types in banana by using a SCAR marker. Aust J Crop Sci, 1(1): 47-52.

Titov, S., S.K. Bhowmik, A. Mandal, M.S. Alam, and S.N. Uddin. 2006. Control of phenolic compound secretion and effect of growth regulators for organ formation from Musa spp. cv. Kanthali floral bud explants. Am. J. Biochem. Biotechnol, 2(3): 97-104.

Uma, S., S. Lakshmi, M.S. Saraswathi, A. Akbar, and M.M. Mustaffa. 2012. Plant regeneration through somatic embryogenesis from immature and mature zygotic embryos of Musa acuminata ssp. burmannica. In Vitro Cellular & Developmental Biology-Plant, 48(5): 539-545.

Xiao, W., X.L. Huang, X. Huang, Y.P. Chen, X.M. Dai, and J.T. Zhao. 2007. Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 90(2): 191-200.

Yudha, H., S. Rahayu, and S. Hanum. 2015. Barangan banana (Musa acuminata L.) shoots induction by application of NAA and BA based on the sources of explant basal. Jurnal Biosains, 1(2): 13-18.



  • There are currently no refbacks.

Copyright (c) 2020 Quagga: Jurnal Pendidikan dan Biologi

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

QUAGGA : Jurnal Pendidikan dan Biologi

ISSN 1907-3089 (print), ISSN 2651-5869 (online)

Organized by Biological Education Study Program, Faculty of Teachers Training and Education, Universitas Kuningan, Indonesia.

Website :

Email :

Address : Jalan Cut Nyak Dhien No.36A Kuningan, Jawa Barat, Indonesia.

View My Stats

Lisensi Creative Commons
Ciptaan disebarluaskan di bawah Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.